Lab activities for Spring 2008, Block 3
Click here to go block 4 schedule
| Week - Date | Lab Activity |
| Weeks 1-7 | Block 3 |
| Week 1 (Jan. 7-10) | No lab |
| Week 2 (Jan. 14-17) | Introduction to Lab Drosophila mapping: P cross Ames test (mutagen analysis using Salmonella ): make reagents |
| Week 3 (Jan. 21-24) | Drosophila mapping: check flies Ames test: Set up trial experiment with known mutagens Have individual experimental design approved Plasmid DNA analysis: Purify plasmids from Escherichia coli Reading assignments: Ames test handout QIAprep miniprep kit protocol/handout |
| Week 4 (Jan. 28-31) | Drosophila F1 Cross Ames test: Analyze trial experiment results Set up individual experiments Plasmid DNA analysis: Agarose gel electrophoresis of plasmid DNA |
| Week 5 (Feb. 4-7) | Drospophila mapping: check flies Ames test: Continue individual experiments Practice workshop on writing Materials and Methods, Results, and Conclusions Gene regulation: Escherichia coli lac operon, part 1 Reading assignment: lac operon handout |
| Week 6 (Feb. 11-14) | Drosophila mapping: F2 analysis and fly homogenization Ames test: Scientific paper due Gene regulation: Escherichia coli lac operon, part 2 |
| Week 7 (Feb. 18-21) | Drosophila mapping: F2 analysis and fly homogenization Plasmid DNA analysis: Restriction digests and agarose gel electrophoresis |
Last update: 4/17/08